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KMID : 0545120100200091295
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 9 p.1295 ~ p.1299
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Biosynthesis of Plant-Specific Flavones and Flavonols in Streptomyces venezuelae
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Park Sung-Ryeol
Paik Ji-Hye
Park Je-Won
Yoon Yeo-Joon
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Abstract
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Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone 3β-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols.
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KEYWORD
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flavones, flavonols, heterologous production, Streptomyces venezuelae
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